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onearray oligonucleotide arrays  (Phalanx Biotech)

 
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    Phalanx Biotech onearray oligonucleotide arrays
    Microarray profiling of polysomal fractions isolated from control siRNA- and GYS1 siRNA-treated cells. (A) HeLa cells were transfected with siRNAs and separated on gradients as shown in Figure 4B. Sucrose gradient fractions 8–9, 10–11, and 12–13 were pooled and are indicated as light, medium and heavy polysomes, respectively (see Figure 4B). PolyA-selected RNA was prepared from the gradient input fraction and the various polysomal fractions. Human whole genome <t>oligonucleotide</t> arrays were performed in triplicate on each sample and normalized and averaged on <t>OneArray</t> platforms, performed by Phalanx Biotech. (Palo Alto, CA). Samples from cells transfected with GYS1 siRNAs were compared to samples from cells transfected with control siRNAs. Cluster analysis of genes with a differential p-value of <0.1 was performed. The scale of the heat-map is presented as log2 fold-change. RNAs were categorized into four classes: (1) mRNA whose abundance was diminished in both input and polysomal samples, (2) mRNA whose abundance was diminished in the input sample, but enhanced in heavy polysomal samples, (3) mRNA whose abundance was enhanced in the input sample, but diminished in heavy polysomal fraction, (4) mRNA whose abundance was enhanced in both input and polysomal samples. Several mRNAs displaying significant changes within each of those four classes were chosen for further analysis. (B) Quantitation of selected mRNAs. Changes in total mRNA abundance of selected mRNAs by Northern blot analysis (red bars), compared to the microarray results (blue bars) of the input sample are shown.
    Onearray Oligonucleotide Arrays, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/onearray+oligonucleotide+arrays/pmc03131224-348-23-17?v=Phalanx+Biotech
    Average 90 stars, based on 1 article reviews
    onearray oligonucleotide arrays - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Proteomic Analysis of Ribosomes: Translational Control of mRNA populations by Glycogen Synthase GYS1"

    Article Title: Proteomic Analysis of Ribosomes: Translational Control of mRNA populations by Glycogen Synthase GYS1

    Journal: Journal of molecular biology

    doi: 10.1016/j.jmb.2011.04.064

    Microarray profiling of polysomal fractions isolated from control siRNA- and GYS1 siRNA-treated cells. (A) HeLa cells were transfected with siRNAs and separated on gradients as shown in Figure 4B. Sucrose gradient fractions 8–9, 10–11, and 12–13 were pooled and are indicated as light, medium and heavy polysomes, respectively (see Figure 4B). PolyA-selected RNA was prepared from the gradient input fraction and the various polysomal fractions. Human whole genome oligonucleotide arrays were performed in triplicate on each sample and normalized and averaged on OneArray platforms, performed by Phalanx Biotech. (Palo Alto, CA). Samples from cells transfected with GYS1 siRNAs were compared to samples from cells transfected with control siRNAs. Cluster analysis of genes with a differential p-value of <0.1 was performed. The scale of the heat-map is presented as log2 fold-change. RNAs were categorized into four classes: (1) mRNA whose abundance was diminished in both input and polysomal samples, (2) mRNA whose abundance was diminished in the input sample, but enhanced in heavy polysomal samples, (3) mRNA whose abundance was enhanced in the input sample, but diminished in heavy polysomal fraction, (4) mRNA whose abundance was enhanced in both input and polysomal samples. Several mRNAs displaying significant changes within each of those four classes were chosen for further analysis. (B) Quantitation of selected mRNAs. Changes in total mRNA abundance of selected mRNAs by Northern blot analysis (red bars), compared to the microarray results (blue bars) of the input sample are shown.
    Figure Legend Snippet: Microarray profiling of polysomal fractions isolated from control siRNA- and GYS1 siRNA-treated cells. (A) HeLa cells were transfected with siRNAs and separated on gradients as shown in Figure 4B. Sucrose gradient fractions 8–9, 10–11, and 12–13 were pooled and are indicated as light, medium and heavy polysomes, respectively (see Figure 4B). PolyA-selected RNA was prepared from the gradient input fraction and the various polysomal fractions. Human whole genome oligonucleotide arrays were performed in triplicate on each sample and normalized and averaged on OneArray platforms, performed by Phalanx Biotech. (Palo Alto, CA). Samples from cells transfected with GYS1 siRNAs were compared to samples from cells transfected with control siRNAs. Cluster analysis of genes with a differential p-value of <0.1 was performed. The scale of the heat-map is presented as log2 fold-change. RNAs were categorized into four classes: (1) mRNA whose abundance was diminished in both input and polysomal samples, (2) mRNA whose abundance was diminished in the input sample, but enhanced in heavy polysomal samples, (3) mRNA whose abundance was enhanced in the input sample, but diminished in heavy polysomal fraction, (4) mRNA whose abundance was enhanced in both input and polysomal samples. Several mRNAs displaying significant changes within each of those four classes were chosen for further analysis. (B) Quantitation of selected mRNAs. Changes in total mRNA abundance of selected mRNAs by Northern blot analysis (red bars), compared to the microarray results (blue bars) of the input sample are shown.

    Techniques Used: Microarray, Isolation, Control, Transfection, Quantitation Assay, Northern Blot



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    onearray oligonucleotide arrays  (Phalanx Biotech)
    90
    Phalanx Biotech onearray oligonucleotide arrays
    Microarray profiling of polysomal fractions isolated from control siRNA- and GYS1 siRNA-treated cells. (A) HeLa cells were transfected with siRNAs and separated on gradients as shown in Figure 4B. Sucrose gradient fractions 8–9, 10–11, and 12–13 were pooled and are indicated as light, medium and heavy polysomes, respectively (see Figure 4B). PolyA-selected RNA was prepared from the gradient input fraction and the various polysomal fractions. Human whole genome <t>oligonucleotide</t> arrays were performed in triplicate on each sample and normalized and averaged on <t>OneArray</t> platforms, performed by Phalanx Biotech. (Palo Alto, CA). Samples from cells transfected with GYS1 siRNAs were compared to samples from cells transfected with control siRNAs. Cluster analysis of genes with a differential p-value of <0.1 was performed. The scale of the heat-map is presented as log2 fold-change. RNAs were categorized into four classes: (1) mRNA whose abundance was diminished in both input and polysomal samples, (2) mRNA whose abundance was diminished in the input sample, but enhanced in heavy polysomal samples, (3) mRNA whose abundance was enhanced in the input sample, but diminished in heavy polysomal fraction, (4) mRNA whose abundance was enhanced in both input and polysomal samples. Several mRNAs displaying significant changes within each of those four classes were chosen for further analysis. (B) Quantitation of selected mRNAs. Changes in total mRNA abundance of selected mRNAs by Northern blot analysis (red bars), compared to the microarray results (blue bars) of the input sample are shown.
    Onearray Oligonucleotide Arrays, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/onearray+oligonucleotide+arrays/pmc03131224-348-23-17?v=Phalanx+Biotech
    Average 90 stars, based on 1 article reviews
    onearray oligonucleotide arrays - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Microarray profiling of polysomal fractions isolated from control siRNA- and GYS1 siRNA-treated cells. (A) HeLa cells were transfected with siRNAs and separated on gradients as shown in Figure 4B. Sucrose gradient fractions 8–9, 10–11, and 12–13 were pooled and are indicated as light, medium and heavy polysomes, respectively (see Figure 4B). PolyA-selected RNA was prepared from the gradient input fraction and the various polysomal fractions. Human whole genome oligonucleotide arrays were performed in triplicate on each sample and normalized and averaged on OneArray platforms, performed by Phalanx Biotech. (Palo Alto, CA). Samples from cells transfected with GYS1 siRNAs were compared to samples from cells transfected with control siRNAs. Cluster analysis of genes with a differential p-value of <0.1 was performed. The scale of the heat-map is presented as log2 fold-change. RNAs were categorized into four classes: (1) mRNA whose abundance was diminished in both input and polysomal samples, (2) mRNA whose abundance was diminished in the input sample, but enhanced in heavy polysomal samples, (3) mRNA whose abundance was enhanced in the input sample, but diminished in heavy polysomal fraction, (4) mRNA whose abundance was enhanced in both input and polysomal samples. Several mRNAs displaying significant changes within each of those four classes were chosen for further analysis. (B) Quantitation of selected mRNAs. Changes in total mRNA abundance of selected mRNAs by Northern blot analysis (red bars), compared to the microarray results (blue bars) of the input sample are shown.

    Journal: Journal of molecular biology

    Article Title: Proteomic Analysis of Ribosomes: Translational Control of mRNA populations by Glycogen Synthase GYS1

    doi: 10.1016/j.jmb.2011.04.064

    Figure Lengend Snippet: Microarray profiling of polysomal fractions isolated from control siRNA- and GYS1 siRNA-treated cells. (A) HeLa cells were transfected with siRNAs and separated on gradients as shown in Figure 4B. Sucrose gradient fractions 8–9, 10–11, and 12–13 were pooled and are indicated as light, medium and heavy polysomes, respectively (see Figure 4B). PolyA-selected RNA was prepared from the gradient input fraction and the various polysomal fractions. Human whole genome oligonucleotide arrays were performed in triplicate on each sample and normalized and averaged on OneArray platforms, performed by Phalanx Biotech. (Palo Alto, CA). Samples from cells transfected with GYS1 siRNAs were compared to samples from cells transfected with control siRNAs. Cluster analysis of genes with a differential p-value of <0.1 was performed. The scale of the heat-map is presented as log2 fold-change. RNAs were categorized into four classes: (1) mRNA whose abundance was diminished in both input and polysomal samples, (2) mRNA whose abundance was diminished in the input sample, but enhanced in heavy polysomal samples, (3) mRNA whose abundance was enhanced in the input sample, but diminished in heavy polysomal fraction, (4) mRNA whose abundance was enhanced in both input and polysomal samples. Several mRNAs displaying significant changes within each of those four classes were chosen for further analysis. (B) Quantitation of selected mRNAs. Changes in total mRNA abundance of selected mRNAs by Northern blot analysis (red bars), compared to the microarray results (blue bars) of the input sample are shown.

    Article Snippet: RNA was pelleted and washed as above and RNA amplification and microarrays were performed in triplicate by Phalanx Biotech (Palo Alto, CA) using OneArray oligonucleotide arrays.

    Techniques: Microarray, Isolation, Control, Transfection, Quantitation Assay, Northern Blot